Publications(1998 - 2017)

[2017] [2016] [2015] [2014] [2013] [2012] [2011]

[2010] [2009] [2008] [2007] [2006] [2005] [2004] [2003] [2002] [2001]

[2000] [1999] [1998]




Blaß LK, Christian Weyler C, Heinzle E (2017)

Network Design and Analysis for Multi-Enzyme Biocatalysis

BMC Bioinformatics, 18:366. DOI 10.1186/s12859-017-1773-y


Richa Garg, Elmar Heinzle, Fozia Noor (2017)

Hepatocytes of Wistar and Sprague Dawley Rats Differ Significantly in Their Central Metabolism

J Cellular Biochem, DOI: 10.1002/jcb.26255


Orsini M, Sperber S, Noor F, Hoffmann E, Weber SN, Hall RA, Lammert F, Heinzle E (2017)

Proteomic characterization of primary mouse hepatocytes in collagen monolayer and sandwich culture

J Cellular Biochem, DOI: 10.1002/jcb.26202


Weyler C, Heinzle E (2017)
Synthesis of Natural Variants and Synthetic Derivatives of the Cyclic Nonribosomal Peptide Luminmide in Permeabilized E. coli Nissle and Product Formation Kinetics

Appl Microbiol Biotechnol 101:131–138. DOI: 10.1007/s00253-016-7770-7.


Ben Yahia B, Gourevitch B, Malphettes L, Heinzle E (2017)

Segmented linear modelling of CHO fed-batch culture and its application to large scale production

Biotechnol Bioeng, 114:985-797. DOI: 10.1002/bit.26214


Wittwehr C, Aladjov H, Ankley G, Byrne HJ, de Knecht J, Heinzle E, Klambauer G, Landesmann B, Luijten M, MacKay C, Gavin Maxwell G, Meek ME, Paini A, Perkins E, Sobanski T, Villeneuve D, Watersa KM, Whelan M (2017)

How Adverse Outcome Pathways Can Aid the Development and Use of Computational Prediction Models for Regulatory Toxicology

Toxicol Sci  155: 326-336, doi: 10.1093/toxsci/kfw207.


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Richter LH, Kaminski YR, Noor F, Meyer MR, Maurer HH (2016)

Metabolic fate of desomorphine elucidated using rat urine, pooled human liver preparations, and human hepatocyte cultures as well as its detectability using standard urine screening approaches.

Anal Bioanal Chem, 408:6283-94. doi: 10.1007/s00216-016-9740-4.


Weltin A, Hammer S, Noor F, Kaminski Y, Kieninger J, Urban GA (2016)

Accessing 3D microtissue metabolism: Lactate and oxygen monitoring in hepatocyte spheroids

Biosens Bioelectron. 87:941-948. doi: 10.1016/j.bios.2016.07.094.


CW Hung, Klein T, Cassidy L, Linke D, Lange S, Anders U, Bureik M, Heinzle E, Schneider K, Tholey A (2016)

Comparative Proteome Analysis in Schizosaccharomyces pombe Identifies Metabolic Targets to Improve Protein Production and Secretion.

Molecular and Cellular Proteomics, 15:3090-3106.


Priesnitz C, Sperber S, Garg R, Orsini M, Noor F (2016)
Fluorescence based cell counting in collagen monolayer cultures of primary hepatocytes. Cytotechnol, 68, 1647–1653


Klein S, Maggioni S, Bucher J, Mueller D, Niklas J, Shevchenko V, Mauch K, Heinzle E, Noor F (2016)

In silico modeling for the prediction of dose and pathway related adverse effects in humans from in vitro repeated-dose studies

Tox Sci, 149, 55–66, doi: 10.1093/toxsci/kfv218.


Leite SB, Roosens T, El Taghdouini A, Mannaerts I, Smout AJ, Najimi M, Sokal E, Noor F, Chesne C, van Grunsven LA (2016)

Novel human hepatic organoid model enables testing of drug-induced liver fibrosis in vitro. Biomaterials. 78:1-10. doi: 10.1016/j.biomaterials.2015.11.026.


Weyler C, Bureik M, Heinzle E (2016)
Selective oxidation of UDP-glucose to UDP-glucuronic acid using permeabilized Schizosaccharomyces pombe expressing human UDP-glucose 6-dehydrogenase.

Biotechnol Lett. 38:477-81. doi: 10.1007/s10529-015-1995-x.


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Noor F* (2015)

Cholestatic-liver diseases: moving from animal models to human biology based systems.

J Physiol, 593:5043-5055.


Krug S, Hollemeyer K, Unger A, Simon S, Parzinger H, Molodin VI (2015)

Die Haaranalysen aus dem Skythengrab Olon-Kurin-Gol 10, Kurgan 1

In. Berliner Beiträge zur Archäometrie, Kunstechnologie und Konservierungswissenschaft, Ed. I. Reiche, Rathgen-Forschungslabor, Staatliche Museen zu Berlin – Stiftung Preußischer Kulturbesitz., Bd. 23, p.85-102


Nicolae N, Wahrheit J, Nonnenmacher Y, Weyler C, Elmar Heinzle E (2015)

Identification of active elementary flux modes in mitochondria using selectively permeabilized CHO cells

Metabol Eng 32:95–105.


Ben Yahia B, Malphettes L, Heinzle E (2015)

Macroscopic modeling of mammalian cell growth and metabolism.

Appl Microbiol Biotechnol, DOI: 10.1007/s00253-015-6743-6.


Weyler C, Heinzle E (2015)
Multistep Synthesis of UDP-Glucose Using Tailored Permeabilized Cells of E. coli

Appl Biochem Biotechnol, 175:3729-3736. DOI 10.1007/s12010-015-1540-3


Klein T, Heinzle E, Schneider K (2015)

Acetate-containing substrate mixtures improve recombinant protein secretion in Schizosaccharomyces pombe

Eng Life Sci, DOI: 10.1002/elsc.201400192


Krauser S, Hoffmann T, Heinzle E (2015)

Directed Multistep Biocatalysis for the Synthesis of the Polyketide Oxytetracycline in Permeabilized Cells of Escherichia coli

ACS Catal 2015:1407−1413, DOI: 10.1021/cs501825u


Wahrheit J, Nonnenmacher Y, Sperber S, Heinzle E (2015)
High-throughput respiration screening of single mitochondrial substrates using permeabilized CHO cells highlights control of mitochondria metabolism

Eng Life Sci, 15:184–194, DOI: 10.1002/elsc.201400175


Klein T, Niklas J, Heinzle E (2015)

Engineering the supply chain for protein production/secretion in yeasts and mammalian cells.

J Ind Microbiol Biotechnol, 42:453–464. DOI 10.1007/s10295-014-1569-2


Miranda-Santos I, Gramacho S, Pineiro M, Martinez-Gomez K, Fritz M, Hollemeyer K, Salvador A, Heinzle E (2015)

Mass isotopomer analysis of nucleosides isolated from RNA and DNA using GC-MS

Anal Chem, 87:617-623.


Gocht T, Berggren E, Ahr HJ, Cotgreave I, Cronin MTD, Daston G, Hardy B, Heinzle E, Hescheler J, Knight DJ, Mahony C, Peschanski M, Schwarz M, Russell S. RS, Verfaillie C, White A, Whelan M (2015)

The SEURAT-1 Approach towards Animal Free Human Safety Assessment

Altex 32(1), 2015.


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Priesnitz C, Sperber S, Garg R, Orsini M, Noor F (2014)

Fluorescence based cell counting in collagen monolayer cultures of primary hepatocytes

Cytotechnol, DOI 10.1007/s10616-014-9821-1


Alépée N, Bahinski T, Daneshian M, De Wever B, Fritsche E, Goldberg A, Hansmann J, Hartung T, Haycock J, Hogberg H, Hoelting L, Kelm JM, Kadereit S, McVey E, Landsiedel R, Leist M, Lübberstedt M, Noor F, Pellevoisin C, Petersohn D, Pfannenbecker U, Reisinger K, Ramirez T, Rothen-Rutishauser B, Schäfer-Korting M, Zeilinger K, Zurich MG.

State-of-the-art of 3D cultures (organs-on-a-chip) in safety testing and pathophysiology.

ALTEX. 2014 Jul 14. doi:


Nicolae A, Wahrheit J, Bahnemann J, Zeng AP, Heinzle E (2014)
Non-stationary 13C metabolic flux analysis of Chinese hamster ovary cells in batch culture using extracellular labeling highlights metabolic reversibility and compartmentation

BMC Sys Biol, 2014, 8:50 doi:10.1186/1752-0509-8-50


Wahrheit J, Niklas J, Heinzle E (2014)

Metabolic control at the cytosol-mitochondria interface in different growth phases of CHO cells

Metabol Eng, 23:9-12. 10.1016/j.ymben.2014.02.001.


Mueller D, Krämer L, Hoffmann E, Klein S, Noor F. (2014)

3D organotypic HepaRG cultures as in vitro model for acute and repeated dose toxicity studies.

Toxicol In Vitro. 28:104-12. doi: 10.1016/j.tiv.2013.06.024.


Wahrheit J, Nicolae A, Heinzle E (2014)

Dynamics of growth and metabolism controlled by glutamine availability in Chinese hamster ovary cells

Appl Microbiol Biotechnol. 98:1771-83. DOI 10.1007/s00253-013-5452-2


Klein T, Lange S, Wilhelm N, Bureik M, Yang TH, Heinzle E, Schneider K (2014)

Overcoming the Metabolic Burden of Protein Secretion in Schizosaccharomyces pombe – A Quantitative Approach using 13C-based Metabolic Flux Analysis

Metabol Eng, 21:34–45,


Wahrheit J, Heinzle E. (2014)
Quenching methods for the analysis of intracellular metabolites.

Methods Mol Biol. 1104:211-21. doi: 10.1007/978-1-62703-733-4_14.


Klein S, Mueller D, Schevchenko V, Noor F. (2014)

Long-term maintenance of HepaRG cells in serum-free conditions and application in a repeated dose study.

J Appl Toxicol. 34:1078-86 Sep 30. doi: 10.1002/jat.2929.


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Wahrheit J, Nicolae A, Heinzle E (2013)
Investigation of glutamine metabolism in CHO cells by dynamic metabolic flux analysis

BMC Proceedings, 7(Suppl 6):P44.


Ramirez T, Daneshian M, Kamp H, Bois FY, Clench MR, Coen M, Donley B, Fischer SM, Ekman DR, Fabian E, Guillou C, Heuer J, Hogberg HT, Jungnickel H, Keun HC, Krennrich G, Krupp E, Luch A, Noor F, Peter E, Riefke B, Seymour M, Skinner N, Smirnova L, Verheij E, Wagner S, Hartung T, van Ravenzwaay B, Leist M. (2013)

Metabolomics in toxicology and preclinical research.

ALTEX. 30:209-25.


Krauser S, Weyler C, Blaß LK, Heinzle E. (2013)

Directed multistep biocatalysis using tailored permeabilized cells.

Adv Biochem Eng Biotechnol. 137:185-234. doi: 10.1007/10_2013_240.


Wahrheit J, Nicolae A, Heinzle E (2013)
13C labeling dynamics of intra- and extracellular metabolites in CHO suspension cells
BMC Proceedings, 7(Suppl 6):P43.


Wahrheit J, Heinzle E (2013)
Sampling and quenching of CHO suspension cells for the analysis of intracellular metabolites

BMC Proceedings, 7(Suppl 6):P42.


Bahnemann J, Kayo S, Wahrheit J, Heinzle E, Pörtner R, Zeng AP (2013)

In search of an effective cell disruption method to isolate intact mitochondria from Chinese hamster ovary cells

Eng Life Sci , 14:161-69, DOI: 10.1002/elsc.201200182


Castillo R, Heinzle E, Peifer S, Schneider K, Pena MCF (2013)

Oxygen supply strongly influences metabolic fluxes, the production of poly(3-hydroxybutyrate) and alginate, and the degree of acetylation of alginate in Azotobacter vinelandii

Proc Biochem, 48:995–1003. doi: 10.1016/j.procbio.2013.04.014


Freund S, Rath A, Platas Barradas O, Skerhutt E, Scholz, S, Niklas J, Sandig V, Rose T, Heinzle E, Noll T, Pörtner R, Zeng AP, Reichl U (2013)

Batch-to-batch variability of two human designer cell lines AGE1.HN and AGE1.HN.AAT carried out by different laboratories under defined culture conditions using a mathematical model

Eng Life Sci, 13:580-92. doi: 10.1002/elsc.201200111


Gunness P, Mueller E, Shevchenko V, Heinzle E, Ingelman-Sundberg M, Noor F (2013)
3D organotypic cultures of human HepaRG cells: a tool for in vitro toxicity studies

Tox Sci, 133:67-78. doi:10.1093/toxsci/kft021.


Mueller D, Heinzle E, Noor F (2013)

3D Hepatic In Vitro Models as Tools for Toxicity Studies

Current Tissue Eng, 2:78-89. DOI: 10.2174/2211542011302010007


Klein T, Heinzle E, Schneider K (2013)
Metabolic fluxes in Schizosaccharomyces pombe grown on glucose and mixtures of glycerol and acetate

Appl Microbiol Biotechnol, 97:5013-26. DOI: 10.1007/s00253-013-4718-z.


Niklas J, Priesnitz C, Rose T, Sandig V, Heinzle E (2013)

Metabolism and metabolic burden by α1-antitrypsin production in human AGE1.HN cells

Metabol Eng, 16:103–114. DOI: 10.1016/j.ymben.2013.01.002


Müller D., Heinzle E. (2013)
Stable isotope assisted metabolomics to detect metabolic flux changes in mammalian cell cultures.
Curr Opin Biotechnol, 24:54–59, DOI: 10.1016/j.copbio.2012.10.015 


Klein T., Schneider K., Heinzle E. (2013)

A system of miniaturized stirred bioreactors for parallel continuous cultivation of yeast with online measurement of dissolved oxygen and off-gas

Biotechnol Bioeng, 110:535-42. doi: 10.1002/bit.24633.


Mueller D., Tascher G., Damm G., Nüssler AK., Heinzle E., Noor F. (2013)

Real-time in situ viability assessment in 3D bioreactor with liver cells using resazurin assay
Cytotechnol, 65:297-305. doi: 10.1007/s10616-012-9486-6.

Neuner A., Wagner, I., Sieker T., Ulber R., Schneider K., Peifer, S., Heinzle E. (2013)
Production of L-Lysine on Different Silage Juices Using Genetically Engineered C. glutamicum

J Biotechnol, 163:217-2410. 1016/j.jbiotec.2012.07.190




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Peifer  S., Barduhn  T., Zimmet  S., Volmer D.A., Heinzle  E., Schneider  K. (2012)

Metabolic engineering of the purine biosynthetic pathway in Corynebacterium glutamicum results in increased intracellular pool sizes of IMP and hypoxanthine

Microbial Cell Factories 11:138. DOI: 10.1186/1475-2859-11-138


Soromani C, Zeng N, Hollemeyer K, Heinzle E, Klein MC, Tretter, T, Seaman MNJ, Roemisch K (2012)
N-acetylation and phosphorylation of Sec complex subunits in the ER membrane

BMC Cell Biology 13:34


Peifer S., Schneider K., Nürenberg G., Volmer D.A., Heinzle E. (2012)
Quantitation of intracellular purine intermediates in different Corynebacteria using electrospray LC-MS/MS
Anal Bioanal Chem, 404:2295-2305.
DOI 10.1007/s00216-012-6388-6.


Krejčík Z, Schleheck D, Hollemeyer K, Cook AM (2012)

A five-gene cluster involved in utilization of taurine-nitrogen and excretion of sulfoacetaldehyde by Acinetobacter radioresistens SH164.

Arch Microbiol. 194:857-63.


Schneider K., Dorscheid S., Witte K., Giffhorn F., Heinzle E. (2012)
Controlled feeding of hydrogen peroxide as oxygen source improves production of 5-ketofructose from L-sorbose using engineered pyranose 2-oxidase from Peniophora gigantea

Biotechnol Bioeng, 109:2941-5. DOI 10.1002/bit.24572.


Mayer J, Denger K, Hollemeyer K, Schleheck D, Cook AM (2012)

(R)-Cysteate-nitrogen assimilation by Cupriavidus necator H16 with excretion of 3-sulfolactate: a patchwork pathway.

Arch Microbiol, 194:949-57. DOI:10.1007/s00203-012-0825-y


Klein S., Heinzle, E. (2012)

Isotope labeling experiments in metabolomics and fluxomics

WIREs Syst Biol Med 4:261-72. doi: 10.1002/wsbm.1167


Hollemeyer K, Altmeyer W, Heinzle E, Pitra C. (2012)

MALDI-TOF Mass Spectrometry combined with multidimensional Scaling, binary hierarchical Cluster Tree and selected diagnostic Masses improves Species Identification of Neolithic Keratin Sequences from Furs of the Tyrolean Iceman Oetzi

Rapid Commun Mass Spectrom, 26:1735–1745


Mueller D., Müller-Vieira U., Biemel K., Tascher G., Nussler AK. and Noor F. (2012)

Biotransformation of diclofenac and effects on the metabolome of primary human hepatocytes upon repeated dose exposure

Eur J of Pharm Sci, 45:716–724


Krauser S, Kiefer P, Heinzle E (2012)

Multi-Enzyme Whole-Cell In Situ Biocatalysis for the Production of Flaviolin in Permeabilized Cells of Escherichia coli

ChemCatChem 4:786-788. DOI: 10.1002/cctc.201100351


Priesnitz C, Niklas J, Rose T, Sandig V, Heinzle E (2012)
Metabolic flux rearrangement in the amino acid metabolism reduces ammonia stress in the α1-antitrypsin producing human AGE1.HN cell line

Metabol Eng, 14:128–137. doi: 10.1016/j.ymben.2012.01.001


Niklas J, Nonnenmacher Y, Rose T, Sandig V, Heinzle E (2012)
Quercetin treatment changes fluxes in the primary metabolism and increases culture longevity and recombinant α1-antitrypsin production in human AGE1.HN cells

Appl Microbiol Biotechnol, 94:57-67. DOI 10.1007/s00253-011-3811-4.


Kuhn D, Julsing MK, Heinzle E, Bühler B (2012)

Systematic optimization of a biocatalytic two-liquid phase oxyfunctionalization process guided by ecological and economic assessment.

Green Chem, 14:645 – 653. DOI:10.1039/C2GC15985F.


Strigun A, Wahrheit J, Niklas J, Heinzle E, Noor F (2012)

Doxorubicin increases oxidative metabolism in HL-1 cardiomyocytes as shown by 13C- metabolic flux analysis
Tox Sci, 125:595-606. doi: 10.1093/toxsci/kfr298


Niklas J, Heinzle E (2012)

Metabolic Flux Analysis in Systems Biology of Mammalian Cells

Adv Biochem Eng Biotechnol,  127:109-132. DOI: 10.1007/10_2011_99


Niklas J, Priesnitz C, Rose T, Sandig V, Heinzle E (2012)
Primary metabolism in the new human cell line AGE1.HN at various substrate levels: increased metabolic efficiency and α1-antitrypsin production at reduced pyruvate load

Appl Microbiol Biotechnol, 93:1637–1650, DOI: 10.1007/s00253-011-3526-6.


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Mueller D, Koetemann A, Noor F (2011)

Organotypic cultures of HepG2 cells for in vitro toxicity studies

J Bioeng Biomed Sci, S2:002, DOI: 10.4172/2155-9538.S2-002


Denger K, Huhn T, Hollemeyer K, Schleheck D, Cook AM (2011)

Sulfoquinovose degraded by pure cultures of bacteria with release of C3-organosulfonates: complete degradation in two-member communities.

FEMS Microbiol Lett 328:39-45. DOI:10.1111/j.1574-6968.2011.02477.x


Schneider K (2011)

Non-Invasive Optical Dissolved Oxygen Quantification in Small-Scale Bioreactors.

Innovations Pharm Technol, 39:14-18.


Fritsch K, Hollemeyer K (2011)

Verbreitung spätkeltischer Importamphoren im Umfeld des keltischen Oppidums “Hunnenring” bei Otzenhausen (Kr. St. Wendel, Saarland, BRD)
Bull. Soc. Préhist. Luxembourgeoise 33:75-108


Niklas J, Hollemeyer K, Heinzle E (2011)

High throughput phospholipid quantitation in mammalian cells using MALDI-TOF MS with N-trifluoroacetyl-phosphatidylethanolamine as internal standard

Anal Biochem, 419:351–353, DOI: 10.1016/j.ab.2011.08.030.


Wahrheit J, Nicolae A, Heinzle E (2011)

Eukaryotic metabolism: Measuring compartment fluxes.

Biotechnol J, 6:1071-85. doi: 10.1002/biot.201100032.


Niklas J, Sandig V, Heinzle E (2011)
Metabolite Channeling and Compartmentation in the Human Cell Line AGE1.HN Determined by 13C Labeling Experiments and 13C Metabolic Flux Analysis

J Biosci Bioeng, 112:616–623, 201110.1016/j.jbiosc.2011.07.021.


Wahrheit J, Niklas J, Heinzle E (2011)

Evaluation of sampling and quenching procedures for the analysis of intracellular metabolites in CHO suspension cells

BMC Proceedings 5(Suppl 8):P82


Niklas J, Priesnitz C, Rose T, Sandig V, Heinzle E (2011)

Producer vs. parental cell – metabolic changes and burden upon a1-antitrypsin production in AGE1.HN®

BMC Proceedings 5(Suppl 8):P85


Sieker T, Neuner A, Dimitrova D, Tippkötter N, Muffler K, Bart H-J, Heinzle E, Ulber R (2011)

Ethanol production from grass silage by simultaneous pretreatment, saccharification and fermentation: First steps in the process development

Eng Life Sci, 11:436 - 442


Schräder E, Scholz, S, Niklas J, Rath A, Platas Barradas O, Jandt U, Sandig V, Rose T, Pörtner R, Reichl U, An-Ping Zeng AP, Heinzle E, Noll T (2011)
Characterisation of cultivation of the human cell line AGE1.HN.AAT

BMC Proceedings 5(Suppl 8):P87


Niklas J, Nonnenmacher Y, Rose T, Sandig V, Heinzle E (2011)

Compartmentation and channelling of metabolites in the human cell line AGE1.HN®

BMC Proceedings 5(Suppl 8):P84


Le Guevel X, Hotzer B, Gregor Jung G, Hollemeyer K, Trouillet V, Schneider M (2011)

Formation of Fluorescent Metal (Au, Ag) Nanoclusters Capped in Bovine Serum Albumin Followed by Fluorescence and Spectroscopy

J Phys Chem C, 115:10955-10963.   DOI: 10.1021/jp111820b


Niklas J, Melnyk A, Yuan Y, Heinzle E (2011)
Selective permeabilization for the high-throughput measurement of compartmented enzyme activities in mammalian cells

Anal Biochem, 416: 218-227. DOI: 10.1016/j.ab.2011.05.039.


Mandenius CF, Andersson TB, Alves PM, Batzl-Hartmann C, Björquist P, Carrondo MJ, Chesne C, Coecke S, Edsbagge J, Fredriksson JM, Gerlach JC, Heinzle E, Ingelman-Sundberg M, Johansson I, Küppers-Munther B, Müller-Vieira U, Noor F, Zeilinger K. (2011).

Toward Preclinical Predictive Drug Testing for Metabolism and Hepatotoxicity by Using In Vitro Models Derived from Human Embryonic Stem Cells and Human Cell Lines - A Report on the Vitrocellomics EU-project.

Altern Lab Anim. 39:147-71


Mueller D, Tascher G, Müller-Vieira U, Knobeloch D, Nuessler AK, Zeilinger K, Heinzle E, Noor F (2011)

In-depth physiological characterization of primary human hepatocytes in a 3D-hollow fiber bioreactor

J Tissue Eng Regen Med, 5: e207-e218, DOI: 10.1002/term.418


Strigun A, Noor F, Pironti A, Niklas J, Tang TH, Heinzle E (2011)

Metabolic flux analysis gives an insight on verapamil-induced changes in central metabolism of HL-1 cells.

J Biotechnol, 155: 299– 307.


Neuner A, Heinzle E (2011)

Mixed Glucose and Lactate Uptake by Corynebacterium glutamicum through Metabolic Engineering

Biotechnology J, 6:318-29. doi: 10.1002/biot.201000307.

Strigun A, Wahrheit J, Beckers S, Heinzle E, Noor F (2011)

Metabolic profiling using HPLC allows classification of drugs according to their mechanisms of action in HL-1 cardiomyocytes

Toxicol Appl Pharmacol 252:183-91. doi:10.1016/j.taap.2011.02.008.


Mandenius CF, Steel D, Noor F, Meyer T, Heinzle E, Asp J, Arain S, Kraushaar U, Bremer S, Class R, Sartipy P (2011)

Cardiotoxicity testing using pluripotent stem cell derived human cardiomyocytes and state-of-the-art bioanalytics
J Appl Toxicol, DOI 10.1002/jat.1663


Niklas J, Schräder E, Sandig V, Noll T, Heinzle E (2011)

Quantitative characterization of metabolism and metabolic shifts during growth of the new human cell line AGE1.HN using time resolved metabolic flux analysis

Bioproc Biosys Eng, 34:533-45. DOI : 10.1007/s00449-010-0502-y


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Krejcik Z, Hollemeyer K, Smits THM, Cook AM (2010)

Isethionate formation from taurine in Chromohalobacter salexigens: purification of sulfoacetaldehyde reductase

Microbiology 156:1547–1555


Weinitschke S, Hollemeyer K, Kusian B, Bowien B, Smits TH, Cook AM (2010) Sulfoacetate Is Degraded via a Novel Pathway Involving Sulfoacetyl-CoA and Sulfoacetaldehyde in Cupriavidus necator H16.

J Biol Chem 285:35249-54.


Mayer J, Huhn T, Habeck M, Denger K, Hollemeyer K, Cook AM (2010)

2,3-Dihydroxypropane-1-sulfonate degraded by Cupriavidus pinatubonensis JMP134: purification of dihydroxypropanesulfonate 3-dehydrogenase.

Microbiol.  156:1556-64.


Kuhn D, Kholiq MA, Heinzle E, Bühler B, Schmid A (2010)
Intensification and economic and ecological assessment of a biocatalytic oxyfunctionalization process

Green Chem., 12, 815-27, DOI: 10.1039/b921896c.


Bracewell D, Gernaey KV, Glassey J, Hass VC, Heinzle E, Mandenius C-F, Olsson I-M, Racher A, Staby A, Titchener-Hooker N (2010)
Education and training for measurement, monitoring, modeling & control (M3C) in biochemical engineering: Workshop report and recommendation
Biotechnol J 5:359–367, biot.201000031


Sieker T, Neuner A, Dimitrova D, Tippkötter N, Bart H-J, Heinzle E, Ulber R (2010)

Grassilage als Rohstoff für die chemische Industrie

Chem Ing Tech 82, 1153-1159


Yuan Y, Yang TH, Heinzle E (2010)
13C Metabolic Flux Analysis for Larger-Scale Cultivation Using Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometry

Metabol Eng, 12:392-400, doi:10.1016/j.ymben.2010.02.001.


Schneider K, Schütz V, John GT, Heinzle E (2010)

Optical Device for Parallel Online Measurement of Dissolved Oxygen and pH in Shake Flask Cultures

Bioproc Biosyst Eng 33:541–547. DOI 10.1007/s00449-009-0367-0.


Niklas J, Schneider K, Heinzle E (2010)
Metabolic Flux Analysis in Eukaryotes

Curr Opin Biotechnol 21:63–69. 10.1016/j.copbio.2010.01.011


Beckers S, Noor F, Müller-Vieira U, Mayer M, Strigun A, Heinzle E (2010)

High throughput, non invasive and dynamic toxicity screening on adherent cells using respiratory measurements

Toxicology in Vitro 24:686–694. doi:10.1016/j.tiv.2009.04.018.


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Yuan Y, Heinzle E (2009)
Permeabilization of Corynebacterium glutamicum for NAD(P)H-dependent intracellular enzyme activity measurement

Comptes rendus – Chimie, 12:1154-1162, DOI 10.1016/j.crci.2009.09.006


Niklas J, Noor F, Heinzle E (2009)

Effects of drugs in subtoxic concentrations on the metabolic fluxes in human hepatoma cell line Hep G2.

Toxicol Appl Pharmacol. 240:327-36. DOI 10.1016/j.taap.2009.07.005.

Lasaosa M, Jakoby T, Delmotte N, Huber C, Heinzle E, Tholey A (2009)

Rapid selection of peptide containing fractions in off-line two-dimensional HPLC in shotgun proteome analysis by screening with MALDI TOF MS.
Proteomics. 9:4577-81.


Selevsek N, Rival S, Tholey A, Heinzle E, Heinz U, Hemmingsen L, Adolph HW (2009)

Zinc ion-induced domain organization in metallo-&[beta]-lactamases: A flexible “zinc arm” for rapid metal ion transfer?

J Biol Chem, 284:16419-31. DOI 10.1074/jbc.M109.001305.

Delmotte N, Lasaosa M, Tholey A, Heinzle E, van Dorsselaer A, Huber CG (2009)

Repeatability of peptide identifications in shotgun proteome analysis employing off-line two-dimensional chromatographic separations and iontrap MS

J Sep Sci 32:1156 – 1164

Noor F, Niklas J, Müller-Vieira U, Heinzle E (2009)

An integrated approach to improved toxicity prediction for the safety assessment

during preclinical drug development using Hep G2 cells.

Toxicol Appl Pharmacol 237:221–231. doi:10.1016/j.taap.2009.03.011

Yang TH, Bolten CJ, Coppi MV, Sun J, Heinzle E (2009)

Numerical Bias Estimation for MS Mass Isotopomer Analysis.

Anal Biochem. 388: 192-203.

Lasaosa M, Delmotte N, Huber CG, Melchior K, Heinzle E, Tholey A (2009)
A two-dimensional reversed-phase x ion-pair reversed-phase HPLC MALDI TOF/TOF MS approach for shotgun proteome analysis.

Anal Bioanal Chem, 393:1245–1256. DOI 10.1007/s00216-008-2539-1

Schneider K, Krömer JO, Wittmann C, Alves-Rodrigues I, Meyerhans A, Diez J, Heinzle E (2009)

Metabolite profiling studies in Saccharomyces cerevisiae: an assisting tool to prioritize host targets for antiviral drug screening.

Microbial Cell Factories, 8:12, doi:10.1186/1475-2859-8-12


Bolten CJ, Heinzle E, Müller R, Wittmann C (2009)

Investigation of the central carbon metabolism of Sorangium cellulosum - Metabolic Network Reconstruction and Quantification of Pathway Fluxes.

J Microbiol Biotechnol, 19:23-36.


Pföhler C, Hollemeyer K, Heinzle E, Altmeyer W, Graeber S, Müller C, Stark A, Jager S, Tilgen W (2009)

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS): a new tool in diagnostic investigation of nail disorders?

Exp Dermatol, 18:880-2.

Deshpande RR, Heinzle  E (2009)

Online monitoring of oxygen in spinner flasks
Biotechnol Letters, 31:665.  DOI: 10.1007/s10529-009-9919-2.

Deshpande RR, Yang TH, Heinzle E (2009)

Towards metabolic and isotopic steady state in CHO batch cultures for reliable isotope-based metabolic profiling
Biotechnol J 4:247-263. DOI: 10.1002/biot.200800143.


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Krejcík Z, Denger K, Weinitschke S, Hollemeyer K, Paces V, Cook AM, Smits TH (2008)

Sulfoacetate released during the assimilation of taurine-nitrogen by Neptuniibacter caesariensis: purification of sulfoacetaldehyde dehydrogenase

Arch Microbiol, 190:159-68. doi: 10.1007/s00203-008-0386-2.

Hollemeyer K, Altmeyer W, Heinzle E, Pitra C. (2008)

Species identification of Oetzi's clothing with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry based on peptide pattern similarities of hair digests.

Rapid Commun Mass Spectrom 22:2751-67.

Krömer JO, Bolten DJ, Heinzle E, Schröder H, Wittmann C (2008)
Physiological response of Corynebacterium glutamicum to oxidative stress induced by deletion of the transcriptional repressor McbR.
Microbiol 154:3917-30.

Denger K, Mayer J, Hollemeyer K, Cook A (2008)

Amphoteric surfactant N-oleoyl-N-methyltaurine utilized by Pseudomonas alcaligenes with excretion of N-methyltaurine.

FEMS Microbiology Letters 288:112-7.


Bolten C, Wittmann C (2008)

Appropriate sampling for intracellular amino acid analysis in five phylogenetically different yeasts
Biotechnol. Lett. 30:1993-2000.


Reitz S, Alhapel A, Essen LO, Pierek AJ (2008)

Structural and Kinetic Properties of a b-Hydroxyacid Dehydrogenase Involved in Nicotinate Fermentation.
J Mol Biol 382, 802–811 doi:10.1016/j.jmb.2008.07.050.


Mayer J, Denger K, Kaspar K, Hollemeyer K, Smits THM, Huhn T, Cook AM (2008)

Assimilation of homotaurine-nitrogen by Burkholderia sp. and excretion of sulfopropanoate

FEMS Microbiol Lett 279:77-82. doi:10.1111/j.1574-6968.2007.01014.x


Heinzle E, Yuan Y, Kumar S, Wittmann C, Gehre M, Richnow H-H, Wehrung P, Adam P, Albrecht P (2008)

Analysis of 13C Labeling Enrichment in Microbial Culture Applying Metabolic Tracer Experiments Using Gas Chromatography-Combustion-Isotope Ratio Mass Spectrometry.

Anal Biochem 380:202-10. doi:10.1016/j.ab.2008.05.039.


Schultheiss E, Weiss E, Winterer E, Maas R, Heinzle E, Jose J (2008)

Esterase autodisplay: enzyme engineering and whole cell activity determination in microplates with pH-sensors.

Appl Environ Microbiol, 74:4782-91.


Fonseca GG, Heinzle E, Wittmann C, Gombert AK (2008)

The yeast Kluyveromyces marxianus and its biotechnological potential.

Appl Microbiol Biotechnol, 79:339-54. DOI 10.1007/s00253-008-1458-6.


Yang TH, Frick O, Heinzle  E (2008)

Hybrid optimization for 13C metabolic flux analysis using systems parametrized by compactification
BMC Systems Biology 2:29.


Wittmann C, Heinzle E (2008)

Metabolic Network Analysis and Design in Corynebacterium glutamicum. In: Corynebacteria. Genomics and Molecular Biology (Ed. A. Burkovski) Caister Academic Press, Norfolk UK

ISBN: 978-1-904455-30-1, p. 79-112.


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Becker J, Klopprogge C, Herold A, Zelder O, Bolten CJ, Wittmann C (2007)

Metabolic flux engineering of l-lysine production in Corynebacterium glutamicum—over expression and modification of G6P dehydrogenase

J Biotechnol 132:99-109


Schneiker S, Perlova O, Kaiser O, Gerth K, Alici A, Altmeyer MO, Bartels D, Bekel T, Beyer S, Bode E, Bode HB, Bolten CJ, Choudhuri JV, Doss S, Elnakady YA, Frank B, Gaigalat L, Goesmann A, Groeger C, Gross F, Jelsbak L, Jelsbak L, Kalinowski J, Kegler C, Knauber T, Konietzny S, Kopp M, Krause L, Krug D, Linke B, Mahmud T, Martinez-Arias R, McHardy AC, Merai M, Meyer F, Mormann S, Muñoz-Dorado J, Perez J, Pradella S, Rachid S, Raddatz G, Rosenau F, Rückert C, Sasse F, Scharfe M, Schuster SC, Suen G, Treuner-Lange A, Velicer GJ, Vorhölter FJ, Weissman KJ, Welch RD, Wenzel SC, Whitworth DE, Wilhelm S, Wittmann C, Blöcker H, Pühler A, Müller R. (2007)

Complete genome sequence of the myxobacterium Sorangium cellulosum.

Nat Biotechnol. 25:1281-9.

Liénard BM, Selevsek N, Oldham NJ, Schofield CJ (2007)

Combined Mass Spectrometry and Dynamic Chemistry Approach to Identify Metalloenzyme Inhibitors.

ChemMedChem 2:175-179

Delmotte N, Lasaosa M, Tholey A, Heinzle E, Huber C (2007)

Two-dimensional reversed-phase x ion-pair reversed-phase HPLC: an alternative approach to high-resolution peptide separation for shotgun proteome analysis
J Proteome Res 6:4363-73.

Velagapudi VR, Wittmann C, Schneider K, Heinzle E (2007)
Metabolic Flux Screening of Saccharomyces cerevisiae Single Knockout Strains on Glucose and Galactose Supports Elucidation of Gene Function.

J Biotechnol 132:395–404.


Ingham J, Dunn IJ, Heinzle E, Prenosil JE, Snape JB (2007)

Chemical Engineering Dynamics


Schilling O, Frick O, Herzberg C, Ehrenreich A, Heinzle E, Wittmann C, Stulke J. (2007)

Transcriptional and metabolic responses of Bacillus subtilis to the availability of organic acids: transcription regulation is important but not sufficient to account for metabolic adaptation.

Appl Environ Microbiol 73:499-507.

Wittmann C, Becker J (2007)

The L-Lysine Story: From Metabolic Pathways to Industrial Production

Microbiol Monogr. 5:39-70.

Hollemeyer K, Altmeyer W, Heinzle E (2007)

Identification of Furs of Domestic Dog, Raccoon Dog, Rabbit and Domestic Cat by Hair Analysis using MALDI –TOF Mass Spectrometry.

Spectroscopy Europe 19(2):8-15.

Heinzle, E, Yang TH, Deshpande R (2007)

Analysis and Design of Metabolic Networks – Experiments and Computer Simulation.

17th European Symposium on Computer Aided Process engineering – ESCAPE 17.

Plesu V., Agachi, PS (Eds.). Elsevier, Amsterdam.


Bolten C, Kiefer P, Letisse F, Portais JC, Wittmann C (2007)

Towards appropriate sampling for metabolome analysis of microorganisms.

Anal. Chem. 79:3843-3849.


Wittmann C (2007)

Fluxome Analysis using GC-MS. 

Microb. Cell Fact. 6:6.


Hollemeyer K, Velagapudi VR, Wittmann C, Heinzle E (2007)

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for metabolic flux analyses using isotope-labeled ethanol.
Rapid Commun.Mass Spectrom. 21, 336-342.

Heinzle E, Matsuda F, Miyagawa H, Wakasa K, Nishioka T ( 2007)

Estimation of metabolic fluxes, expression levels and metabolite dynamics of a secondary metabolic pathway in potato using label pulse feeding experiments combined with kinetic network modeling and simulation

The Plant J, 50, 176-87.

Fonseca G, Gombert A, Heinzle E, Wittmann, C. (2007)

Physiology of the yeast Kluyveromyces marxianus during batch and chemostat cultures with glucose as the sole carbon source

FEMS Yeast Research, 7, 422–435.

Heinzle, E, Biwer A, Cooney CL (2007)

Development of Sustainable Bioprocesses – Modeling and Assessment. John Wiley & Sons, Chichester.

Homepage - Book

Fürch, T., R. Hollmann, C. Wittmann, W. Wang and W. D. Deckwer (2007)

Comparative study on central metabolic fluxes of Bacillus megaterium strains in continuous culture using 13C labelled substrates. Bioproc. Biosyst. Eng. 230:47-59.


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Stephan S, Heinzle E, Wenzel SC, Krug D, Müller R, Wittmann C (2006)

Metabolic physiology of Pseudomonas putida for heterologous production of myxochromide.

Process Biochem. 41:2146–2152.

Mayer J, Denger K, Smits THM, Hollemeyer K, Groth U, Cook A (2006)

N-Acetyltaurine dissimilated via taurine by Delftia acidovorans NAT

Archives Microbiol. 186:61-7.


Velagapudi VR, Wittmann C, Lengauer T, Talwar P, Heinzle E (2006)

Metabolic Screening of Saccharomyces cerevisiae Single Knockout Strains Reveals Unexpected Mobilization of Metabolic Potential.

Process Biochem. 41:2170-79.

Tholey A, Heinzle E (2006)

Ionic (liquid) matrices for matrix assisted laser desorption/ionization mass spectrometry - applications and perspectives.

Anal. Bioanal. Chem., 386:24-37.


Tholey A. (2006)

Ionic liquid matrices with phosphoric acid as matrix additive for the facilitated analysis of phosphopeptides by matrix assisted laser desorption/ionization mass spectrometry.

Rapid Commun.Mass Spectrom. 20:1761-1768.


Tholey A (2006)
MALDI Mass Spectrometry for Quantitative Proteomics – Approaches, Scopes and Limitations. 
Dagstuhl Seminar Proceedings 05471 (2005): Computational Proteomics. C. Huber, K. Reinhardt & O. Kohlbacher (Eds.).
Published online: portals/index.php?semnr=05471.


Selevsek N, Liénard B, Tholey A, Heinzle E, Heinz U, Adolph H-W, Frère J-M, Oldham NJ, Schofield CJ (2006)

Studies on Ternary Metallo-ß-lactamase-inhibitor Complexes Using Electrospray Ionization Mass Spectrometry

J Am Soc Mass Spectrom 17:1000-1004.


Yang TH, Wittmann C, Heinzle E (2006)
Respirometric 13C Flux Analysis: Part I: Design, Construction and Validation of a Novel Multiple Reactor System using On-line Membrane Inlet Mass Spectrometry

Metabol. Eng., 8, 417-431.


Yang TH, Wittmann C, Heinzle E (2006)
Respirometric 13C Flux Analysis: Part II: In vivo Flux Estimation of Lysine-producing Corynebacterium glutamicum.

Metabol. Eng., 8, 432-446.


Weinitschke S, Denger K, Smits THM, Hollemeyer K, Cook AM (2006)

The sulfonated osmolyte N-methyltaurine is dissimilated by Alcaligenes faecalis and by Paracoccus versutus with release of methylamine.
Microbiology 152:1179-1186.


Abdul Kholiq M, Heinzle E (2006)

Prozessmodellierung und Nachhaltigkeitsbewertung einer Ganzzellbiotransformation im zweiphasigen System ionische Flüssigkeit/Wasser

Chemie Ing. Tech., 78:307-316.


Heinzle E, Biwer A, Eissen M, Abdul Kholiq M (2006)

Bewertung biotechnologischer Prozesse in frühen Phasen der Entwicklung hinsichtlich Risiken bezüglich Ökologie, Sicherheit und Gesundheit.

Chemie Ing. Tech., 78:301-305.


Fürch T, Hollmann R, Wang W, Wittmann C, Deckwer W-D (2006)

Dynamische Untersuchungen zum Aminosäure-Stoffwechsel von Bacillus megaterium mittels stabiler Isotope

Chem Ing Tech 78, 295-300

Kroemer JO, Wittmann C, Schroeder H, Heinzle E (2006)

Metabolic Pathway Analysis for Rational Design of L-Methionine Production by Escherichia coli and Corynebacterium glutamicum

Metabol.Eng., 8, 353-369.

Kim HM, Heinzle E, Wittmann C. (2006)

Deregulation of aspartokinase by single nucleotide exchange leads to global flux rearrangement in the central metabolism of Corynebacterium glutamicum.
J. Microbiol. Biotechnol. 16:1174-1179

Kroemer JO, Heinzle E, Schroeder H, Wittmann C (2006)

Accumulation of Homolanthionine and Activation of a Novel Pathway for Isoleucine Biosynthesis in Corynebacterium glutamicum McbR Deletion Strains.

J Bacteriol, 188:609-618

Tholey A, Zabet-Moghaddam M, Heinzle E. (2006)

Quantification of Peptides for the Monitoring of Protease-Catalyzed Reactions by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Ionic Liquid Matrixes

Anal. Chem. 78:291-297

Zabet-Moghaddam, M., Heinzle, E., Lasaosa, M., Tholey, A. (2006)

Pyridinium-based ionic liquid matrices can improve the identification of proteins by peptide mass-fingerprint analysis with matrix-assisted laser desorption/ionization mass spectrometry.

Anal Bioanal Chem 384:215–224

Krömer J, Heinzle E, Wittmann C (2006)
Quantification of S-adenosyl methionine in microbial cell extracts.
Biotechnol. Lett. 28:69-71


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Wittmann, C., Hans, M., van Winden, W., Ras, C. & Heijnen, S. (2005)
Dynamics of intracellular metabolites of glycolysis and TCA cycle during cell-cycle related oscillation in Saccharomyces cerevisiae.
Biotechnol. Bioeng., 89:839-47.

Frick, O., C. Wittmann (2005)
Characterization of the metabolic shift between oxidative and fermentative growth in Saccharomyces cerevisiae by comparative 13C flux analysis.
Microb. Cell. Factories. 4:30-46.


Wittmann, C., De Graaf, A.A. (2005)
Metabolic Flux Analysis in  Corynebacterium glutamicum. In: Handbook of Corynebacterium glutamicum (Eds. L. Eggeling, M. Bott) Taylor & Francis, Boca Raton , p. 277 – 304.


Arain S, Weiss S, Heinzle E, John GT, Krause C, Klimant I. (2005)
Gas sensing in microplates with optodes: influence of oxygen exchange between sample, air, and plate material.
Biotechnol Bioeng. 90:271-80.


Ilvesniemi, H., Kähkönen, M. A., Pumpanen, J., Rannik, Ü., Wittmann, C., Perämäki, M., Keronen, P., Hari, P., Vesala, T. & Salkinoja-Salonen, M. 2005
Wintertime CO2 evolution from a boreal forest ecosystem.

Boreal Env. Res. 10: 401–408.


Kurola, J, Wittmann, C., Aarnio, T., Tsitko, I., Salkinoja-Salonen, M., Romantschuk, M. (2005).

Application of cation exchange membranes for detecting ammonia-oxidising bacteria in soils by fluorescence in situ hybridization (FISH).
FEMS Microbiol. Ecol. 53:463-472.


Becker J, Klopprogge C, Zelder O, Heinzle E, Wittmann C (2005)

Amplified Expression of Fructose 1,6-bisphosphatase in Corynebacterium glutamicum increases in vivo flux through the pentose phosphate pathway and lysine production on different carbon sources.

Appl. Env. Microbiol. 71:8587–8596.


Heinzle E (2005)

Metabolic Engineering. In: Angewandte Mikrobiologie (Ed. G. Antranikian)

Springer, Berlin ISBN-10 3-450-24083-7.


Tholey A, Toll H, Huber CG (2005)

Separation and detection of phosphorylated and non-phosphorylated peptides in liquid chromatography-mass spectrometry using monolithic columns and acidic or alkaline mobile phases

Anal. Chem., 77:4618-4625


Biwer AP, Zuber PT, Zelic B, Gerharz T, Bellmann KJ, Heinzle E (2005)

Modeling and analysis of a new process for pyruvate production.

Ind.Eng.Chem.Res. 44: 3124-3133.


Rein U, Gueta R, Denger K, Ruff J, Hollemeyer K, Cook A. (2005)
Dissimilation of cysteate via 3-sulfolactate sulfo-lyase and a sulphate exporter in Paracoccus pantotrophus NKNCYSA.

Microbiology, 151:737-47.


Hollemeyer K, Jager S, Altmeyer W, Heinzle E. (2005)
Proteolytic peptide patterns as indicators for fungal infections and nonfungal affections of human nails measured by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.

Anal Biochem. 338:326-31.


Yang TH, Heinzle E, Wittmann C (2005)
Theoretical Aspects of 13C Metabolic Flux Analysis with Sole Quantification of Carbon Dioxide Labeling.
Comput. Biol. Chem. 29:121-133

Deshpande RR, Kirsch Y, Maas R, John GT, Krause C, Heinzle E (2005)

Microplates with Integrated Oxygen Sensors for Kinetic Cell Respiration Measurement and Cytotoxicity Testing in Primary and Secondary Cell Lines

ASSAY Drug Devel. Technol. 3: 299-307.

Kromer JO, Fritz M, Heinzle E, Wittmann E. (2005)

In vivo quantification of intracellular amino acids and intermediates of the methionine pathway in Corynebacterium glutamicum.

Anal. Biochem. 340: 171-173.

Heinz U, Kiefer M, Tholey A, Adolph HW (2005)
On the competition for available zinc.
J. Biol. Chem. 280, 3197-3207.


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Deshpande, R.R., Wittmann, C., Heinzle, E. (2004)
Microplates with integrated oxygen sensing for medium optimization in animal cell culture

Cytotechnol. 46:1–8.

Wittmann, C., Schütz, V., John, G., Heinzle, E. (2004)

Quantification of oxygen transfer in test tubes by integrated optical sensing.
J. Microbiol. Biotechnol,  14, 991-995.

Zabet-Moghaddam, M., Krüger, R., Heinzle, E., Tholey, A. (2004)
MALDI mass spectrometry for the characterization of ionic liquids and the analysis of amino acids, peptides and proteins in ionic liquids. 
J. Mass Spectrom., 39: 1494-1505.


Wittmann, C., Kiefer, P., Zelder, O. (2004)

Metabolic Fluxes in Corynebacterium glutamicum during lysine production on sucrose.

Appl. Env. Microbiol., 70: 7277-7287.

Wittmann, C., Heinzle, E. (2004)
Metabolic Activity Profiling by 13C Tracer Experiments and Mass Spectrometry in  Corynebacterium glutamicum. In: Methods in Biotechnology, Vol 18: Microbial Processes and Products (Ed. J.L. Barredo), Huma Press, Totowa, NJ., p. 151-163


Yang, T.H., Wittmann, C., Heinzle, E. (2004)
Membrane Inlet Mass Spectrometry for the On-line Measurement of Metabolic Fluxes in the Case of Lysine-Production by Corynebacterium glutamicum.
Eng. Life Sci. 4:252-257


Yang, T.H., Wittmann, C., Heinzle, E. (2004)
Metabolic Network Simulation Using Logical Loop Algorithm and Jacobian Matrix.
Metab. Eng., 6:256-267.


Denger, K., Weinitschke S., Hollemeyer, K. , Cook, A.M. (2004)
Sulfoacetate generated by Rhodopseudomonas palustris from taurine.
Arch. Microbiol., 182:254-258.


Biwer, A., Heinzle, E. (2004)
Process modeling and simulation can guide process development: Case Study
Enzyme Microbiol. Technol. 34:642-650.


Deshpande, R.R., Heinzle, E. (2004)
On-line Oxygen Uptake Rate and Culture Viability Measurement of Animal Cell Culture Using Microplates with Integrated Oxygen Sensors.
Biotechnol. Lett., 26:763–767.


Kiefer, P., Heinzle E., Zelder, O., Wittmann, C. (2004)
Comparative Metabolic Flux Analysis of Lysine-Producing Corynebacterium glutamicum Cultured on Glucose or Fructose.
Appl. Env. Microbiol.  70:229-39.


Wittmann ,C.,  Kroemer,  J. O., Kiefer, P., Binz , T., Heinzle , E. (2004)
Impact of the cold shock phenomenon on quantification of intracellular metabolites in bacteria.
Anal. Biochem. 327:135-9.


Bungert, D., Bastian, S., Heckmann-Pohl, D., Giffhorn, F., Heinzle, E., Tholey, A. (2004)
Screening of sugar converting enzymes using quantitative MALDI-ToF mass spectrometry
Biotechnol. Lett., 26:125-30.

Krömer, J.O., Sorgenfrei, O., Klopprogge, K., Heinzle, E. , Wittmann, C. (2004)
In-Depth Profiling of Lysine Producing Corynebacterium glutamicum by Combined Analysis of Transcriptome, Metabolome and Fluxome
J. Bacteriol
. 186:1769-84

Bungert, D., Heinzle, E., Tholey, A. (2004)
Quantitative Matrix Assisted Laser Desorption/Ionization Mass Spectrometry for the Determination of Enzyme Activities
Anal. Biochem., 326, 167-175.

Zabet-Moghaddam, M., Heinzle, E., Tholey, A. (2004)
Qualitative and quantitative analysis of low molecular weight compounds by ultraviolet matrix-assisted laser desorption/ionization mass spectrometry using ionic liquid matrices.
Rapid Comm. Mass Spectrom., 18:141-148.

Kumar, S., Wittmann, C., Heinzle, E. (2004)
Review: Minibioreactors
Lett., 26: 1-10.

Wittmann, C., Kim H.M, Heinzle, E. (2004)
Metabolic flux analysis  of lysine producing Corynebacterium glutamicum at miniaturized scale.
Biotechnol. Bioeng., 87:1-6.

Biwer, A., Heinzle, E. (2004)
Environmental Assessment in Early Process Development
J. Chem.
Technol. Biotechnol., 79:597–609

Wittmann, C., Kähkönen, M.,  Ilvesniemi, H., Kurola, J., Salkinoja-Salonen, M.S. (2004)
Areal activities and stratification of hydrolytic enzymes involved in the biochemical cycles of carbon, nitrogen, sulphur, and phosphorous in podsolized forest soils.
Soil Biol. Biochem., in press. 


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Yang, T.H., Wittmann, C., Heinzle, E. (2003)
Dynamic calibration and dissolved gas analysis using membrane inlet mass spectrometry for the quantification of cell respiration.
Rapid Comm. Mass Spectrom. 17,


Wittmann, C., Kim, H.-M., John, G., Heinzle, E. (2003)
Characterization and application of an optical sensor for quantification of dissolved O2 in shake-flasks .
Biotechnol. Lett. 25, 377-380.


John, G., Goelling, D., Klimant, I., Schneider, H., Heinzle, E. (2003)
pH-Sensing 96-well micrtotiter plates for the characterisation of acid production by dairy starter cultures.
J.Dairy Res. 70, 327-333.


John, G.T., Klimant, I., Wittmann, C., Heinzle, E. (2003)
Integrated Optical Sensing of Dissolved Oxygen in Microtiter Plates – A Novel Tool for Microbial Cultivation,
Bioeng. 81, 829-836.


Yang, T.H., Wittmann, C., Heinzle, E. (2003)

Membraneinlass-Massenspektrometrie zur prozessnahen metabolischen Flussanalyse am Beispiel der Lysin-Produktion von Corynebacterium glutamicum

Membrane Inlet Mass Spectrometry for Process-Near Analysis of Metabolic Fluxes: Application to Lysine Producing Corynebacterium glutamicum

Chemie Ing. Tech. 75, 1359-1364.


Mathur, S., Hassel, M., Steiner, F., Hollemeyer, K., Hartmann, R.W. (2003)

Development of a New Approach for Screening Combinatorial Libraries Using MALDI-TOF-MS and HPLC-ESI-MS/MS

J. Biomol. Screening 8, 136-148


Hans, M., Heinzle, E., Wittmann, C. (2003)
Free intracellular amino acid pools during autonomous oscillations in Saccharomyces cerevisiae.
Biotechnol. Bioeng. 82, 143-51.


Dunn, I.J., Heinzle, E., Ingham, J., Prenosil, J.E. (2003)
Biological Reaction Engineering.
Dynamic Modelling Fundamentals with Simulation Exercises.
Wiley-VCH, Weinheim.


Talwar, P., Wittmann, C., Heinzle, E., Lengauer, T. (2003)
A software tool for automated processing of 13C labeling data from mass spectrometric spectra.
Biotechniques, 35, 1214-1215.


Heinzle, E., Deshpande, R. Bock, U., John, G., Krause, C., Räbiger, T., Müller, G., Waltenberger, H., Maas, R. (2003)
Entwicklung von innovativen Mikrotiterplattenreaktoren zur Bewertung des Abbaus und der Toxizität neuer Wirkstoffe auf Basis von Mikrosomen und Säugerzellen (Development of innovative micro-titer plate reactors for the assessment of degradation and toxicity of drugs using microsomes and mammalian cells)
transkript Sonderband Biokatalyse, 66-67.


Heinzle, E., Weiß, S., Wolfbeis, O., Arain, S., Klimant, I., John, G., Krause, C., Räbiger, T., Müller, G., Waltenberger, H., Jose, J., Schultheiss, E. (2003)
Mikrotiterplatten-Reaktoren mit integrierten pH-Sensoren und Autodisplay in E. coli zur evolutiven Enzymentwicklung (Micro-titer plate reactors with integrated pH-Sensors and auto-display in E. coli for the evolutive enzyme development)
transkript Sonderband Biokatalyse, 59-62.


Biwer, A., Zuber, P., Bellmann, K., Sell, D., Gebhart, P. Heinzle, E. (2003)
Ökoeffizienz-Bewertung in der Prozessentwicklung (Assessment of eco-efficiency in the process development)
transkript Sonderband Biokatalyse, 115-118.



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Hollemeyer,K., Heinzle, E., Tholey, A. (2002)
Identification of oxidized methionine residues in peptides containing two methionine residues by derivatization and MALDI-TOF mass spectrometry.
Proteomics 2, 1524-1531.


Hollemeyer, K., Altmeyer, W., Heinzle, E. (2002)
Identification and quantification of feathers, down and hair of avian and mammalian origin using MALDI-TOF- mass spectrometry.
Anal. Chem., 74, 5960-5968, 2002


Wittmann, C., Hans, M., Bluemke, W. (2002)
Metabolic Physiology of Aroma Producing Kluyveromyces marxianus.
Yeast 68, 584


Wittmann, C., Heinzle, E. (2002)
Genealogy profiling through strain improvement using metabolic network analysis – metabolic flux genealogy of several generations of lysine producing corynebacteria.
Appl. Environ. Microbiol. 68, 5843-5859.


Tholey, A., Wittmann, C., Kang, M.-J., Bungert, D., Hollemeyer, K., Heinzle, E. (2002)
Derivatization of small biomolecules for optimized matrix-assisted laser desorption/ionization mass spectrometry.
J. Mass Spectrom. 37, 963-973


Biwer, A., Antranikian G., Heinzle E. (2002)
Mini-Review: Enzymatic production of cyclodextrins.
Appl. Microbiol. Biotechnol. 59, 609-617


Van Winden, W. A., Wittmann, C., Heinzle, E., Heijnen, J.J. (2002).
Correcting mass isotopomer distributions for naturally occurring isotopes.
Biotechnol. Bioeng. 80, 477-479.


Weiß, S., John, G.T., Klimant, I., Heinzle, E. (2002)
Modeling of Mixing in 96-well microplates observed with fluorescence indicators.
Biotechnol. Prog. 18, 821-830.


Kiefer, P., Heinzle, E., Wittmann, C. (2002)
Influence of Glucose, Fructose and Sucrose as Carbon Sources on Kinetics and Stoichiometry of Lysine Production by Corynebacterium glutamicum.
J. Ind. Microbiol. Biotechnol. 28, 338-43.


Wittmann, C., Hans, M., Heinzle, E. (2002)
In vivo analysis of intracellular amino acid labelings by GC/MS.
Anal. Biochem. 307, 379-382.


Biwer, A., Heinzle, E. (2002)
Early ecological evaluation in biotechnology through process simulation: Case study citric acid.
Eng. Life Sci. 2, 265-268.


Kähkönen, M.A., Wittmann, C., Ilvesniemi, H. Westman, C.J., Salkinoja-Salonen, M.S. (2002)
Mineralization of detritus and oxidation of methane in acid boreal coniferous forest soils: Seasonal and vertical distribution and effects of clear-cut.
Soil Biol. Biochem. 34, 1191-1200.


Wittmann, C. (2002)
Metabolic flux analysis using mass spectrometry.
Adv. Biochem. Eng. Biotechnol, 74, 39-64.


Tholey, A. and Heinzle, E. (2002)
Methods for Biocatalyst Screening.
Adv. Biochem. Eng. Biotechnol. 74, 1-19.


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Oezemre, A., Heinzle, E. (2001)
Measurement of oxygen uptake and carbon dioxide production rate of mammalian cells using membrane mass spectrometry.
Cytotechnology 37, 153-162.


Biwer, A., Heinzle, E. (2001)
Ökoeffiziente Prozesse - Beispiele aus der chemischen Industrie.
In: Biotechnologie als interdisziplinäre Herausforderung. Eds. Heiden, St., Burschel, C. und Erb, R. Spektrum Verlag; pp. 191-208.


Biwer, A., Heinzle, E. (2001).
Prozesssimulation zur frühen ökologischen Bewertung biotechnologischer Prozesse: Beispiel Zitronensäure.
Chem.Ing.Tech. 73, 1467-1471.


Biwer, A., Rudershausen, A., Heinzle, E., Bellmann, K., Sell, D., Gebhart, P. (2001)
Ökologische und ökonomische Evaluation biokatalytischer Prozesse während ihrer Entwicklung,
In: Biokatalyse (Eds. S. Heiden and R. Erb), BIOspektrum, Spektrum Akademischer Verlag, Heidelberg, pp. 15 - 24.


Bonarius, H.P.J., Oezemre, A., Timmerarends, B., Skrabal, P., Tramper, J., Schmid, G., Heinzle, E. (2001)
Metabolic-Flux Analysis of continuously cultured hybridoma cells using 13CO2 mass spectrometry in combination with in combination with 13C-lactate NMR spectroscopy and metabolite balancing.
Biotechnol. Bioeng. 74, 528-538.


Hans, M., Heinzle, E., Wittmann, C. (2001)
Quantification of intracellular amino acids in batch-cultures of Saccharomeyces cerevisiae.
Appl. Microbiol. Biotechnol. 56, 776-779.


John, G.T. and Heinzle, E. (2001)
Quantitative screening method for hydrolases in microplates using pH indicators: Determination of kinetic parameters by dynamic pH monitoring.
Biotechnol. Bioeng. 72, 620-627.


Kähkönen, M. A., Wittmann, C., Kurola, J., Ilvesniemi, H., Salkinoja-Salonen, M.S. (2001)
Microbial activity of boreal forest soil in a cold climate.
Boreal Environ. Res. 6, 19-28.


Kang, M.J., Tholey, A., Heinzle, E. (2001)
Application of automated matrix-assisted laser desorption/ionisation time-of-flight- mass spectrometry for the measurement of enzyme activities.
Rapid Commun. Mass Spectrom. 15, 1327-1333.


Speicher A., Hollemeyer K., Heinzle E. (2001)
Rapid detection of chlorinated bisbibenzyls in Bazzania trilobata using MALDI-TOF Mass spectrometry.
Phytochemistry 57, 303-306.


Speicher, A., Hollemeyer, K., Heinzle, E. (2001)
Rapid detection of multiple chlorinated bis(bibenzyls) in bryophyte crude extracts using laser desorption/ionization time-of-flight mass spectrometry.
Rapid Commun. Mass Spectrom. 2001, 15, 124-127.


Tholey, A., Pipkorn, R., Bossemeyer, D., Kinzel, V. & Reed, J. (2001).
Influence of myristoylation, phosphorylation and deamidation on the structural behaviour of the N-terminus of the catalytic subunit of cAMP-dependent protein kinase.
Biochemistry 40, 225-231.


Treitz, G., Maria, G., Giffhorn, F., Heinzle, E. (2001)
Kinetic Model Discrimination via Step-by-Step Experimental and Computational Procedure in the Enzymatic Oxidation of D-Glucose.
J. Biotechnol., 85, 271 - 287.


Weiß, S., Heinzle, E., John, G.T., Klimant, I. (2001)
Sauerstofftransfer und Mischverhalten in 96er Mikrotiterplatten.
Bioforum, 10, 662-664.


Weiß, S., John, G.T., Heinzle, E., Schultheiss, E., Jose, J., Arain, S., Krause, C., Klimant, I., Waltenberger, H., Maier, J., Räbiger, T. (2001)
Screening von Biokatalysatoren mit Sauerstoff- und pH-Mikrotiterplatten,
In: Biokatalyse (Eds. S. Heiden and R. Erb), BIOspektrum, Spektrum Akademischer Verlag, Heidelberg, pp. 54-59.


Wittmann, C. Heinzle, E. (2001)
Novel approach for metabolic flux analysis - application of MALDI-TOF MS to lysine-producing Corynebacterium glutamicum.
Eur. J. Biochem. 268, 2441-2455.


Wittmann, C., Heinzle, E. (2001)
MALDI TOF MS for quantification of substrates and products in cultivations of lysine producing Corynebacterium glutamicum.
Biotechnol. Bioeng. 72, 642-647.


Wittmann, C., Heinzle, E. (2001)
Modeling and experimental design for metabolic flux analysis of lysine-producing corynebacteria by mass spectrometry.
Metabol. Eng. 3, 173-191.


Wittmann, C., Yang, T., Kochems, I., Heinzle, E. (2001)
Dynamic respiratory measurements with Corynebacterium glutamicum using membrane mass spectrometry.
J. Microbiol. Biotechnol., 11, 40-49.


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Ingham, J., Dunn, I.J., Heinzle, E., Prenosil, J.E. (2000)
Chemical Engineering Dynamics.
An Introduction to Modelling and Computer Simulation, 2nd Edition, Wiley-VCH, Weinheim.


Jeworski, M., Heinzle, E. (2000)
Combined chemical-biological treatment of wastewater containing refractory pollutants.
Biotechnol. Ann. Rev., Vol.6. Ed. M. Raafat El-Gewely. Elsevier: Amsterdam, pp.163-196


Kang, M.-J., Tholey, A., Heinzle, E. (2000)
Quantitation of low molecular mass substrates and products fo enzyme ctalyzed reactions using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Rapid Commun. Mass Spectrom. 14, 1972-1978.


Roethlisberger, P., Iida-Tanaka, N., Hollemeyer, K., Heinzle, E., Ishizuka, I., Fischer, W. (2000)
Unique poly(glycerophosphate)lipoteichoic acid and the glycolipids of a Streptococcus sp. closely related to Pneumococcus.
Eur. J. Biochem. 267, 5520-5530.


Wittmann, C., Suominen, K.P. and Salkinoja-Salonen M.S. (2000)
Evaluation of ecological disturbance and intrinsic bioremediation potential of pulp mill contaminated lake sediment using key enzymes as probes.
Environ. Poll. 107, 255-261.


Schlehek, D., Dong, W., Denger, K., Heinzle, E. , Cook, A.M. (2000)
An a-Proteobacterium Converts Linear Alkylbenzenesulfonate (LAS) Surfactants into Sulfophenylcaboxylates, and Linear Alkyldiphenyletherdisulfonate Surfactants into Sulfodiphenylethercarboxylates.
Appl. Environ. Microbiol. 66, 1911-1916.


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Wittmann, C., Heinzle, E. (1999)
Mass Spectrometry for Metabolic Flux Analysis.
Biotechnol.Bioeng. 62, 739-750.


Maria, G., Heinzle, E. (1999)
Testing Novel Schort-Cut Methods for Complex Kinetic Characterisation in Early Safety Assessment of a Chemical Process.
ESCAPE9, May 31-June 2, 1999, Budapest


Heinzle, E. (1999)
Ökologische und ökonomische Beurteilung von Produktionsprozessen in frühen Entwicklungsphasen.
In: Industrielle Nutzung von Biokatalysatoren (Eds. Heiden, S., Bock, A.-K., Antranikian, G.), Erich Schmidt Verlag, Berlin.


Soda, S. Heinzle, E., Fujita, M. (1999)
Modeling and simulation of competition of two microorganisms for a single inhibitory substrate in a biofilm reactor.
Biotechnol. Bioeng. 66, 258-264.


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Meyer, B., Heinzle, E. (1998)
Dynamic Determination of Anaerobic Acetate Kinetics Using Membrane Mass Spectrometry.
Biotechnol. Bioeng. 57, 127-135.


Heinzle, E., Meyer, B., Oezemre, A.,Dunn, I.J. (1998)
A Microreactor with On-line Mass Spectrometry for the Investigation of Biological Kinetics.
In: Microreaction Technology (Ed. W. Ehrfeld), Springer, Berlin, pp. 267-274.


Heinzle, E., Weirich, D., Brogli, F., Hoffmann, V., Koller, G., Verduyn, M.A., Hungerbühler, K. (1998)
Ecological and Economic Objective Functions for Screening in Integrated Development of Fine Chemical Processes.
I. Flexible and Expandable Framework Using Indices.
Ind. Eng. Chem. Res. 37, 3395-3407.


Hungerbühler, K., Heinzle, E., Ranke, J. (1998)
Integrierte Entwicklung chemischer Prozesse.
In: Chemische Produkte und Prozesse. Springer, Berlin. p. 207-227.


Koller, G., Weirich, D., Brogli, F., Heinzle, E., Hoffmann, V., Verduyn, M.A., Hungerbühler, K. (1998)
Ecological and Economic Objective Functions for Screening in Integrated Development of Fine Chemical Processes.
II. Stream Allocation and Case Studies.
Ind. Eng. Chem. Res. 37, 3408-3413.


Maria, G, Heinzle, E. (1998)
Kinetic system identification by using short-cut techniques in early safety assessment of chemical processes,
J. Loss Prev.
Process Ind. 11, 187-206.



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Heinzle E, Furukawa K, Tanner R, Dunn IJ (1983)

“Modelling of sustained oscillations observed in continuous culture of Saccharomyces cerevisiae”.  in: Modelling and Control of Biotechnical Processes (Ed.: A.Halme) Pergamon Press, Oxford, 57  -  65.