Mittwoch, 13. November 2019

HIPS-Talk: "Dynamic organ-on-a-chip plattforms for the recapitulation of human epithelium and their implication in drug development"

organized by the Helmholtz-Institute for Pharmaceutical Research Saarland (HIPS)

Dr. Rodi Abdalkader from the Institute for Integrated Cell-Material Sciences (iCeMS), University Kyoto,  will give a presentation entitled "Dynamic organ-on-a-chip plattforms for the recapitulation of human epithelium and their implication in drug development".


When: Wednesday, November 13, 2019, at 17:00 s.t.
Where: in Blg E8.1, Seminar Room (Ground Floor) 


Host: Dr. Gregor Fuhrmann

There is opportunity to talk with the speaker before the talk. Guests are welcome!

For details and for making appointments please contact:
Dr. Gregor Fuhrmann
Phone: 0681-98806-1500
E-mail: gregor.fuhrmann(at)helmholtz-hips.de

 

Abstract:

Our body contains natural barriers that work as a defense shield against pathogens intrusion. These biological barriers consist of layers of epithelial cells, stroma, extracellular matrices, and immune cells. Each component plays a vital role in the orchestration of the homeostasis of these biological barriers. In drug development, biological barriers have an essential role in the determination of the pharmacokinetic and the toxicological fate of drugs. Therefore, the emulation of these barriers in vitro is of great importance. The current conventional in vitro models are different from the in vivo due to the lack of the essential biological features, including the low expression of key proteins and transporters. Also, drug screening studies are usually done under static conditions. Consequently, these models fail in the prediction of drugs efficacy/ toxicity, leaving a big gap between in vitro experiments and clinical studies.

Recently, organ-on-a-chip technology provides an attractive solution for the recapitulation of a variety of organs. The use of pluripotent stems cells allows us to generate biologically relevant cells needed for the construction of the barriers. Cells can be grown in the microfluidic devices in a 3D manner supported by sufficient niches of extracellular matrices.

However, in reality, most of the epithelium barriers function under mechanical stimuli, fluid dynamics, shear stress, and air-liquid interface environment. Therefore, the re-creation of these dynamic entities is essential for the establishment of biological barriers on-a-chip that are more applicable to drug development.

In this talk, I will present our approach in the development of two biological barriers on-a-chip: 1. Intestine-on-a-chip 2. Cornea-on-a-chip. I will go through the essential characteristic of these chips and their compatibilities for cell culturing and inflammatory disease modelling. Also, I will briefly point out to our attempts to emulate the fluid dynamics in the eye. Finally, I will address the advantage of using metabolomics in integration with these models..