Forschung

Forschungsschwerpunkte

Unsere Arbeitsgruppe arbeitet an Membrantransport-Systemen für die Aufnahme von Eisen und den Export von Fettsäuren über die Hüllmembranen des Chloroplasten.

Obwohl die Integration dieser Transporter im pflanzlichen Stoffwechsel im Allgemeinen recht gut beschrieben ist, ist über die genaue molekulare Funktionsweise noch sehr wenig bekannt. Die Korrelation von Struktur und Funktion der Transporter ist neuartig und ermöglicht daher die Beschreibung von pflanzenspezifischen sowie generellen Eigenschaften intrazellulärer Transportwege.
Dabei richten wir unseren Fokus auf die Aufklärung der pflanzenphysiologischen Rolle, sowie die detaillierte funktionelle Charakterisierung der entsprechenden Transporter.
Die Studien werden an der Modellpflanze Arabidopsis thaliana sowie an Tabak- und Erbsenpflanzen durchgeführt.

Publikationen

2020. Pham, H. D., Polya, S., Müller, B., Szenthe, K., Sagi-Kazar, M., Bankuti, B., Banati, F., Sarvari, E., Fodor, F., Tamas, L., Philippar, K., and Solti, A. 'The developmental and iron nutritional pattern of PIC1 and NiCo does not support their interdependent and exclusive collaboration in chloroplast iron transport in Brassica napus', Planta, 251: 96, doi.org/10.1007/s00425-020-03388-0

2019. Voith von Voithenberg, L., Park, J., Stübe, R., Lux, C., Lee, Y., and Philippar, K. 'A Novel Prokaryote-Type ECF/ABC Transporter Module in Chloroplast Metal Homeostasis', Front Plant Sci, 10: 1264, doi.org/10.3389/fpls.2019.01264

2019. Vigani, G., Solti, I. D. M., Thomine, S. B., and Philippar, K. 'Essential and Detrimental - an Update on Intracellular Iron Trafficking and Homeostasis', Plant Cell Physiol, 60: 1420-39, doi.org/10.1093/pcp/pcz091

2019. Könnel, A., Bugaeva, W., Gügel, I. L., and Philippar, K. 'BANFF: bending of bilayer membranes by amphiphilic alpha-helices is necessary for form and function of organelles (1)', Biochem Cell Biol, 97: 243-56, doi.org/10.1139/bcb-2018-0150

2017. Li-Beisson, Y., Neunzig, J., Lee, Y., and Philippar, K. 'Plant membrane-protein mediated intracellular traffic of fatty acids and acyl lipids', Curr Opin Plant Biol, 40: 138-46, doi.org/10.1016/j.pbi.2017.09.006

2016. Murcha, M. W., Kubiszewski-Jakubiak, S., Teixeira, P. F., Gügel, I. L., Kmiec, B., Narsai, R., Ivanova, A., Megel, C., Schock, A., Kraus, S., Berkowitz, O., Glaser, E., Philippar, K., Marechal-Drouard, L., Soll, J., and Whelan, J. 'Plant-Specific Preprotein and Amino Acid Transporter Proteins Are Required for tRNA Import into Mitochondria', Plant Physiol, 172: 2471-90, doi.org/10.1104/pp.16.01519

2016. Harsman, A., Schock, A., Hemmis, B., Wahl, V., Jeshen, I., Bartsch, P., Schlereth, A., Pertl-Obermeyer, H., Goetze, T. A., Soll, J., Philippar, K., and Wagner, R. 'OEP40, a Regulated Glucose-permeable beta-Barrel Solute Channel in the Chloroplast Outer Envelope Membrane', J Biol Chem, 291: 17848-60, doi.org/10.1074/jbc.M115.712398

2016. Li, N., Xu, C., Li-Beisson, Y., and Philippar, K. 'Fatty Acid and Lipid Transport in Plant Cells', Trends Plant Sci, 21: 145-58, doi.org/10.1016/j.tplants.2015.10.011

2016. Lopez-Millan, A. F., Duy, D., and Philippar, K. 'Chloroplast Iron Transport Proteins - Function and Impact on Plant Physiology', Front Plant Sci, 7: 178, doi.org/10.3389/fpls.2016.00178

2015. Li, N., Gügel, I. L., Giavalisco, P., Zeisler, V., Schreiber, L., Soll, J., and Philippar, K. 'FAX1, a novel membrane protein mediating plastid fatty acid export', PLoS Biol, 13: e1002053, doi.org/10.1371/journal.pbio.1002053

2014. Gutierrez-Carbonell, E., Takahashi, D., Lattanzio, G., Rodriguez-Celma, J., Kehr, J., Soll, J., Philippar, K., Uemura, M., Abadia, J., and Lopez-Millan, A. F. 'The distinct functional roles of the inner and outer chloroplast envelope of Pea (Pisum sativum) as revealed by proteomic approaches', J Proteome Res, 13: 2941-53, doi.org/10.1021/pr500106s

2014. Kleinknecht, L., Wang, F., Stübe, R., Philippar, K., Nickelsen, J., and Bohne, A. V. 'RAP, the sole octotricopeptide repeat protein in Arabidopsis, is required for chloroplast 16S rRNA maturation', Plant Cell, 26: 777-87, doi.org/10.1105/tpc.114.122853

2013. Sudre, D., Gutierrez-Carbonell, E., Lattanzio, G., Rellan-Alvarez, R., Gaymard, F., Wohlgemuth, G., Fiehn, O., Alvarez-Fernandez, A., Zamarreno, A. M., Bacaicoa, E., Duy, D., Garcia-Mina, J. M., Abadia, J., Philippar, K., Lopez-Millan, A. F., and Briat, J. F. 'Iron-dependent modifications of the flower transcriptome, proteome, metabolome, and hormonal content in an Arabidopsis ferritin mutant', J Exp Bot, 64: 2665-88, doi.org/10.1093/jxb/ert112

2013. Vigani, G., Zocchi, G., Bashir, K., Philippar, K., and Briat, J. F. 'Cellular iron homeostasis and metabolism in plant', Front Plant Sci, 4: 490, doi.org/10.3389/fpls.2013.00490

2013. Vigani, G., Zocchi, G., Bashir, K., Philippar, K., and Briat, J. F.  'Signals from chloroplasts and mitochondria for iron homeostasis regulation', Trends Plant Sci, 18: 305-11, doi.org/10.1016/j.tplants.2013.01.006

2012. Pudelski, B., Schock, A., Hoth, S., Radchuk, R., Weber, H., Hofmann, J., Sonnewald, U., Soll, J., and Philippar, K. 'The plastid outer envelope protein OEP16 affects metabolic fluxes during ABA-controlled seed development and germination', J Exp Bot, 63: 1919-36, doi.org/10.1093/jxb/err375

2012. Vogelmann, K., Drechsel, G., Bergler, J., Subert, C., Philippar, K., Soll, J., Engelmann, J. C., Engelsdorf, T., Voll, L. M., and Hoth, S. 'Early senescence and cell death in Arabidopsis saul1 mutants involves the PAD4-dependent salicylic acid pathway', Plant Physiol, 159: 1477-87, doi.org/10.1104/pp.112.196220

2011. Duy, D., Stübe, R., Wanner, G., and Philippar, K. 'The chloroplast permease PIC1 regulates plant growth and development by directing homeostasis and transport of iron', Plant Physiol, 155: 1709-22, doi.org/10.1104/pp.110.170233

2011. Schuler, M., Keller, A., Backes, C., Philippar, K., Lenhof, H. P., and Bauer, P. 'Transcriptome analysis by GeneTrail revealed regulation of functional categories in response to alterations of iron homeostasis in Arabidopsis thaliana', BMC Plant Biol, 11: 87, doi.org/10.1186/1471-2229-11-87

2010. Pudelski, B., Kraus, S., Soll, J., and Philippar, K. 'The plant PRAT proteins - preprotein and amino acid transport in mitochondria and chloroplasts', Plant Biol (Stuttg), 12 Suppl 1: 42-55, doi.org/10.1111/j.1438-8677.2010.00357.x

2009. Benz, J. P., Stengel, A., Lintala, M., Lee, Y. H., Weber, A., Philippar, K., Gügel, I. L., Kaieda, S., Ikegami, T., Mulo, P., Soll, J., and Bölter, B. 'Arabidopsis Tic62 and ferredoxin-NADP(H) oxidoreductase form light-regulated complexes that are integrated into the chloroplast redox poise', Plant Cell, 21: 3965-83, doi.org/10.1105/tpc.109.069815

2009. Pudelski, B., Soll, J., and Philippar, K. 'A search for factors influencing etioplast-chloroplast transition', Proc Natl Acad Sci U S A, 106: 12201-6, doi.org/10.1073/pnas.0902145106

2007. Duy, D., Soll, J., and Philippar, K. 'Solute channels of the outer membrane: from bacteria to chloroplasts', Biol Chem, 388: 879-89, doi.org/10.1515/BC.2007.120

2007. Duy, D., Wanner, G., Meda, A. R., von Wiren, N., Soll, J., and Philippar, K. 'PIC1, an ancient permease in Arabidopsis chloroplasts, mediates iron transport', Plant Cell, 19: 986-1006, doi.org/10.1105/tpc.106.047407

2007. Philippar, K. and Soll, J. 'Intracellular transport: solute transport in chloroplasts, mitochondria, peroxisomes and vacuoles, and between organelles. In Plant Solute Transport, Yeo, A.R. and Flowers, T.J. (eds.)' Oxford, U.K.: Blackwell Publishing, Ltd., pp. 133-192. doi.org/10.1002/9780470988862.ch7

2007. Murcha, M. W., Elhafez, D., Lister, R., Tonti-Filippini, J., Baumgartner, M., Philippar, K., Carrie, C., Mokranjac, D., Soll, J., and Whelan, J. 'Characterization of the preprotein and amino acid transporter gene family in Arabidopsis', Plant Physiol, 143: 199-212, doi.org/10.1104/pp.106.090688

2007. Philippar, K., Geis, T., Ilkavets, I., Oster, U., Schwenkert, S., Meurer, J., and Soll, J. 'Chloroplast biogenesis: the use of mutants to study the etioplast-chloroplast transition', Proc Natl Acad Sci U S A, 104: 678-83, doi.org/10.1073/pnas.0610062104

2006. Fuchs, I., Philippar, K., and Hedrich, R. 'Ion channels meet auxin action', Plant Biol (Stuttg), 8: 353-9, doi.org/10.1055/s-2006-924121

2006. Gerdes, L., Bals, T., Klostermann, E., Karl, M., Philippar, K., Hünken, M., Soll, J., and Schünemann, D. 'A second thylakoid membrane-localized Alb3/OxaI/YidC homologue is involved in proper chloroplast biogenesis in Arabidopsis thaliana', J Biol Chem, 281: 16632-42, doi.org/10.1074/jbc.M513623200

2006. Goetze, T. A., Philippar, K., Ilkavets, I., Soll, J., and Wagner, R. 'OEP37 is a new member of the chloroplast outer membrane ion channels', J Biol Chem, 281: 17989-98, doi.org/10.1074/jbc.M600700200

2006. Philippar, K., Büchsenschütz, K., Edwards, D., Löffler, J., Lüthen, H., Kranz, E., Edwards, K. J., and Hedrich, R. 'The auxin-induced K(+) channel gene Zmk1 in maize functions in coleoptile growth and is required for embryo development', Plant Mol Biol, 61: 757-68, doi.org/10.1007/s11103-006-0047-2

2005. Büchsenschütz, K., Marten, I., Becker, D., Philippar, K., Ache, P., and Hedrich, R. 'Differential expression of K+ channels between guard cells and subsidiary cells within the maize stomatal complex', Planta, 222: 968-76, doi.org/10.1007/s00425-005-0038-6

2004. Clausen, C., Ilkavets, I., Thomson, R., Philippar, K., Vojta, A., Möhlmann, T., Neuhaus, E., Fulgosi, H., and Soll, J. 'Intracellular localization of VDAC proteins in plants', Planta, 220: 30-7, doi.org/10.1007/s00425-004-1325-3

2004. Philippar, K., Ivashikina, N., Ache, P., Christian, M., Lüthen, H., Palme, K., and Hedrich, R. 'Auxin activates KAT1 and KAT2, two K+-channel genes expressed in seedlings of Arabidopsis thaliana', Plant J, 37: 815-27, doi.org/10.1111/j.1365-313x.2003.02006.x

2003. Deeken, R., Ivashikina, N., Czirjak, T., Philippar, K., Becker, D., Ache, P., and Hedrich, R. 'Tumour development in Arabidopsis thaliana involves the Shaker-like K+ channels AKT1 and AKT2/3', Plant J, 34: 778-87, doi.org/10.1046/j.1365-313x.2003.01766.x

2003. Fuchs, I., Philippar, K., Ljung, K., Sandberg, G., and Hedrich, R. 'Blue light regulates an auxin-induced K+-channel gene in the maize coleoptile', Proc Natl Acad Sci U S A, 100: 11795-800, doi.org/10.1073/pnas.2032704100

2003. Philippar, K., Büchsenschütz, K., Abshagen, M., Fuchs, I., Geiger, D., Lacombe, B., and Hedrich, R. 'The K+ channel KZM1 mediates potassium uptake into the phloem and guard cells of the C4 grass Zea mays', J Biol Chem, 278: 16973-81, doi.org/10.1074/jbc.M212720200

2002. Deeken, R., Ache, P., Philippar, K., and Hedrich, R. 'Apoplastic factors regulate the AKT2/3-type potassium channels. In Plant Nutrition, Series: Developments in Plant and Soil Sciences', Springer Netherlands: Kluwer Academic Publishers, Vol. 92, pp. 248-249. doi.org/10.1007/0-306-47624-X_119

2000. Philippar, K., Becker, D., Hedrich, E., Edwards, L., Ljung, S., and Sandberg, G. '. . . response: living with gravity', Trends Plant Sci, 5: 86-7, doi.org/10.1016/s1360-1385(00)01568-5

2000. Bauer, C. S., Hoth, S., Haga, K., Philippar, K., Aoki, N., and Hedrich, R. 'Differential expression and regulation of K(+) channels in the maize coleoptile: molecular and biophysical analysis of cells isolated from cortex and vasculature', Plant J, 24: 139-45, doi.org/10.1046/j.1365-313x.2000.00844.x

1999. Philippar, K., Fuchs, I., Lüthen, H., Hoth, S., Bauer, C. S., Haga, K., Thiel, G., Ljung, K., Sandberg, G., Böttger, M., Becker, D., and Hedrich, R. 'Auxin-induced K+ channel expression represents an essential step in coleoptile growth and gravitropism', Proc Natl Acad Sci U S A, 96: 12186-91, doi.org/10.1073/pnas.96.21.12186