Publications

2024

Reinhard J, Starke L, Klose C, Haberkant P, Hammarén H, Stein F, Klein O, Berhorst C, Stumpf H, Sáenz JP, Hub J, Schuldiner M, Ernst R
MemPrep, a new technology for isolating organellar membranes provides fingerprints of lipid bilayer stress
EMBO J (2024) in press

Ernst R, Renne MF, Jain A, von der Malsburg A.
ER membrane homeostasis and the unfolded protein response
CSH Perspectives (2024) doi:10.1101/cshperspect.a041400

2023

Renne MF, Ernst R.
ERMES - from myths to molecules.
Nature Reviews Molecular Cell Biology (2023)

Renne MF, Ernst R.
Membrane homeostasis beyond membrane fluidity: control of membrane compressibility.
Trends in Biochemical Sciences (2023)

Reinhard J, Leveille CL, Cornell CE, Merz AJ, Klose C, Ernst R, Keller SL.
Remodeling of yeast vacuole membrane lipidomes from the log (1-phase) to stationary stage (2-phase).
Biophysical Journal (2023)

2022

Reinhard J, Starke L, Klose C, Haberkant P, Hammarén H, Stein F, Klein O, Berhorst C, Stumpf H, James P Sáenz, Hub J, Schuldiner M, Ernst R.
A New Technology for Isolating Organellar Membranes Provides Fingerprints of Lipid Bilayer Stress.
bioRxiv, DOI: 10.1101/2022.09.15.508072

Castro I, Shortill SP, Dziurdzik SK, Cadou A, Ganesan S, Fenech EJ, Meyer H, Fadel A, David Y, Davey M, Mattes C, Valenti R, Ernst R,  Zaremberg V, Levine TP, Stefan CJ, Conibear E, Schuldiner M.
Systematic analysis of membrane contact sites in Saccharomyces cerevisiae uncovers modulators of cellular lipid distribution.
eLife (2022) e74602

Alsayyah C, Ernst R.
A Lipid Hydyrolase and a Ubiquitin Ligase Play Hide-and-Seek in the ER Membrane.
The EMBO J (2022) e112384

2021

Radanović T, Ernst R.
The Unfolded Protein Response as a Guardian of the Secretory Pathway.
Cells (2021) 10, 2965

Väth K, Mattes C, Reinhard J, Covino R, Stumpf H, Hummer G, Ernst R.
Cysteine Crosslinking in Native Membranes Establishes the Transmembrane Architecture of Ire1.
Journal of Cell Biology (2021) 220, e202011078

2020

Vabulas M, Martinez-Limón A, Calloni G, Ernst R.
Flavin Dependency Undermines Proteome Stability, Lipid Metabolism and Cellular Proliferation During Vitamin B2 Deficiency.
Cell Death & Disease (2020) 11, 725

Reinhard J, Mattes C, Väth K, Radanović T, Surma MA, Klose C, Ernst R.
A Quantitative Analysis of Cellular Lipid Compositions During Acute Proteotoxic ER Stress
Reveals Specificity in the Production of Asymmetric Lipids.
Frontiers in Cell and Developmental Biology (2020) 8, 756

Levental KR, Malmberg E, Symons JL, Fan YY, Chapkin R, Ernst R, Levental I
Lipidomic and Biophysical Homeostasis of Mammalian Membranes Counteracts Dietary Lipid Perturbations to Maintain Cellular Fitness.
Nature Communications (2020) 11,1339

Ballweg S, Sezgin E, Doktorova M, Covino R, Reinhard J, Wunnicke D, Hänelt I, Levental I, Hummer G, Ernst R
Regulation of Lipid Saturation Without Sensing Membrane Fluidity.
Nature Communications (2020) 11, 756

2018

Covino R, Hummer G, Ernst R
Integrated Functions of Membrane Property Sensors and a Hidden Side of the Unfolded Protein Response.
Molecular Cell (2018) 71, 458-467

Ernst R, Ballweg S, Levental I
Cellular Mechanisms of Physicochemical Membrane Homeostasis.
Current Opinion in Cell Biology (2018) 53, 44-51

Hofbauer HF, Gecht M, Fischer SC, Seybert A, Frangakis AS, Stelzer EHK, Covino R, Hummer G, Ernst R.
The Molecular Recognition of Phosphatidic Acid by an Amphipathic Helix in Opi1.
Journal of Cell Biology (2018) 217, 3109-3126

Radanović T, Reinhard J, Ballweg S, Pesek K, Ernst R
An Emerging Group of Membrane Property Sensors Controls the Physical State of Organellar Membranes to Maintain their Identity.
BioEssays (2018) 40, 1700250

2017

Koop Y, Lang W-H, Schuster T, Martinez-Limon A, Hofbauer H, Ernst R, Calloni G, Vabulas M
CHIP as a Membrane-shuttling Proteostasis Sensor.
eLife (2017) e29388

Cohen N, Breker M, Bakunts A, Pesek K, Chas A, Argemí JM, Orsi A, Gal L, Chuartzman S, Wigelman Y, Jonas F, Walter P, Ernst R,
Aragón T, van Anken E, Schuldiner M
Iron Affects Ire1 Clustering Propensity and the Amplitude of Endoplasmic Reticulum Stress Signaling.
Journal of Cell Science (2017) 130, 3222-3233

Halbleib K, Pesek K, Covino R, Hofbauer HF, Wunnike D, Hänelt I, Hummer G, Ernst R
Activation of the Unfolded Protein Response by Lipid Bilayer Stress.
Molecular Cell (2017) 67, 673-684

Featured by Paulina Strzyz in Nature Reviews Molecular Cell Biology "Reacting to membrane stress"
Featured by Barry Starr on the Saccharomyces genome database "Sensing Disturbances in the Membrane"
Featured in Trends in Cell Biology by T. Aragón and E. van Anken "The Ire1 Twist that Links Proteostatic with Lipostatic Control ..."

Ballweg S, Ernst R
Control of Membrane Fluidity: the OLE Pathway in Focus.
Biological Chemistry (2017) 398, 215-228

2016

Ernst R, Ejsing CS, Antonny B
Homeoviscous Adaptation and the Regulation of Membrane Lipids.
Journal of Molecular Biology (2016) 428, 4776-4791

Covino R, Ballweg S, Stordeur C, Michaelis JB, Puth K, Wernig F, Bahrami A, Ernst AM, Hummer G, Ernst R
A Eukaryotic Sensor for Membrane Lipid Saturation.
Molecular Cell (2016) 63, 49-59

2015

Puth K, Hofbauer HF, Sáenz JP, Ernst R
Homeostatic Control of Biological Membranes by Dedicated Lipid and Membrane Packing Sensors.
Biological Chemistry (2015) 396, 1043-1058

2014

Mehla J, Ernst R, Moore R, Wakschlag A, Marquis MK, Ambudkar SV, Golin J
Evidence for a Molecular Diode-Based Mechanism in a Multispecific ATP-Binding Cassette (ABC) Exporter: SER-1368 as a Gatekeeping Residue in the Yeast Multidrug Transporter Pdr5.
The Journal of Biological Chemistry (2014) 289, 26597-26606

Stordeur C, Puth K, Sáenz JP, Ernst R
Crosstalk of Lipid and Protein Homeostasis to Maintain Membrane Function.
Biological Chemistry (2014) 395, 313-326

2013

Surma MA, Klose C, Peng D, Shales M, Mrejen C, Stefanko A, Braberg H, Gordon DE, Vorkel D, Ejsing CS, Farese R, Simons K, Krogan NJ, Ernst R
A Lipid E-MAP Identifies Ubx2 as a Critical Regulator of Lipid Saturation and Lipid Bilayer Stress.
Molecular Cell (2013) 51, 519-530

2011 - 2012 Postdoctoral work

Ernst R, Claessen JH, Mueller B, Sanyal S, Spooner E, van der Veen AG, Kirak O, Schlieker CD, Weihofen WA, Ploegh HL
Enzymatic Blockade of the Ubiquitin-Proteasome Pathway.
PLoS Biology (2011) 8, e1000605

Featured as Editor`s Choice in Science, Issue 1^st of April 2011 "A Less Toxic Treatment"

Gupta RP, Kueppers P, Schmitt L, Ernst R
The Multidrug Transporter Pdr5: a Molecular Diode?
Biological Chemistry (2011) 392, 53-60

Jumpertz T, Tschapek B, Infed N, Smits SH, Ernst R, Schmitt L
High-Throughput Evaluation of the Critical Micelle Concentration of Detergents.
Analytical Biochemistry (2011) 408, 64-70

Ernst R, Kueppers P, Stindt J, Kuchler K, Schmitt L
Multidrug Efflux Pumps: Substrate Selection in ATP-Binding Cassette Multidrug Efflux Pumps--First Come, First Served?
The FEBS Journal (2010) 277, 540-549

Ernst R, Mueller B, Ploegh HL, Schlieker C
The Otubain YOD1 is a Deubiquitinating Enzyme that Associates with p97 to Facilitate Protein Dislocation from the ER.
Molecular Cell (2009) 36, 28-38

Antos JM, Popp MW, Ernst R, Chew GL, Spooner E, Ploegh HL
A Straight Path to Circular Proteins.
The Journal of Biological Chemistry (2009) 284, 16028-1603

2003 - 2008 PhD work

Ernst R, Kueppers P, Klein CM, Schwarzmueller T, Kuchler K, Schmitt L
A Mutation of the H-Loop Selectively Affects Rhodamine Transport by the Yeast Multidrug ABC Transporter Pdr5.
Proceedings of the National Academy of Sciences of the United States of America – PNAS (2008) 105, 5069-5074

Ernst R, Koch J, Horn C, Tampé R, Schmitt L
Engineering ATPase Activity in the Isolated ABC Cassette of Human TAP1.
The Journal of Biological Chemistry (2006) 281, 27471-27480

Ernst R, Klemm R, Schmitt L, Kuchler K
Yeast ATP-Binding Cassette Transporters: Cellular Cleaning Pumps.
Methods in Enzymology (2005) 400, 460-484

Benabdelhak H, Kiontke S, Horn C, Ernst R, Blight MA, Holland IB, Schmitt L
A Specific Interaction Between the NBD of the ABC-Transporter HlyB and a C-Terminal Fragment of its Transport Substrate Haemolysin A.
Journal of Molecular Biology (2003) 327, 1169-1179